import subprocess PATH_TO_AUTOHOTKEY = r"C:\Program Files\AutoHotkey\AutoHotkey.exe" def eval_authotkey(code): authotkey_process = subprocess.Popen([PATH_TO_AUTOHOTKEY, "*"], shell=True, stdin=subprocess.PIPE, stdout=subprocess.PIPE, stderr=subprocess.PIPE, ) stdout_value, stderr_value = authotkey_process.communicate(code) print(stderr_value) return stdout_value result = eval_authotkey(""" my_var = hello world msgbox % my_var ; Print to stdout: 2 methods ; Method 1 FileAppend line 1`n, * ; Method 2 stdout := FileOpen("*", "w") stdout.WriteLine("line 2") stdout.WriteLine("line 3") """) print (result)